教师名录

【研究方向】

致力于建立高效的猪基因组编辑和功能基因组学分析平台，为生物医学研究和临床前转化提供理想的大动物疾病模型，同时也可以为猪的经济性状改良和品系培育提供新的手段和解决思路。通过化学诱变建立了大规模的突变体，筛选到了影响骨骼发育、肌肉发生、血细胞再生、行为、毛色等猪重要性状的功能基因和突变家系；不但为进一步培育猪新品种提供了育种材料和分子标记，也促进了猪在生物医学中的应用，是动物遗传领域的原创性成果。创建了在猪的乳汁中表达高活性人抗凝血因子9多转基因猪和UCP1基因编辑猪，在提高瘦肉的同时改善了体温调节，为猪的基因编辑育种提供了概念性的验证，获得了国内外权威媒体的正向和广泛报道，主要研究方向为：

1．通过化学诱变进行猪全基因组水平的随机突变和表型分析，建立突变家系，筛选功能基因和培育猪突变体；

2．通过基因组编辑（Genome Editing, GE）技术进行猪的基因组遗传修饰进而改善猪的农业生产性状或构建生物医学研究的大动物模型；

3．猪早期胚胎发育的表观遗传调控和体细胞核移植。

【办公地点】A112

【联系方式】

电话：010-64806259（办公室），13911407503 (手机)

邮箱：zhaojg@ioz.ac.cn

【教育及工作经历】

教育经历：

2000.09-2003.07 博士，东北农业大学动物科技学院动物遗传育种专业，哈尔滨，黑龙江

1997.09-2000.07 硕士，甘肃农业大学动物科技学院动物遗传育种，兰州，甘肃

1993.09-1997.07 本科，青岛农业大学，青岛，山东

科研经历：

2003-2005年，在上海交通大学医学遗传研究所/上海儿童医院任助理研究员；

2005-2007年，在美国新奥尔良大学生物系任博士后研究人员；

2007-2010年，在美国密苏里大学哥伦比亚分校任研究助理教授；

2010-今，中国科学院动物研究所干细胞与生殖生物学国家重点实验室研究员。

【发表文章】

1. Wang XY, Wang Y, Wang Y, Guo YF, Zong RJ, Hu ST, Yue JW, Yao J, Han CS *, Guo JT *, Zhao JG*. Single-cell transcriptomic and cross-species comparison analyses reveal distinct molecular changes of porcine testes during puberty. Communications Biology. 2024,7 (1), 1478

2. Qi YN, Zhang Y, Tian SJ, Zong RJ, Yan XH, Wang Yu, Wang YF *, Zhao JG*. An optimized prime editing system for efficient modification of the pig genome. SCIENCE CHINA Life Sciences. 2023, (66) 2851-2861

3. Ma YJ, Wu BB, Chen YH, Ma S, Wang LY, Han TT, Lin XL, Yang FL, Liu C, Zhao JG*, Li W*. CCDC146 is required for sperm flagellum biogenesis and male fertility in mice. Cellular and Molecular Life Sciences. 2023 Dec 1;81(1):1

4. Dong FC , Wang XG, Huang T , Yin YY, Xiao S, Ma YJ, Wei HF, Wu BB, Zhang RD, Wang LY, Guo XJ, Gao F, Liu C, Liu HB, Zhao JG*, Li W *.CCDC178-mediated cytoskeleton assembly is required for spermiogenesis in mice. Genes &Disease. 2023.09

5. Qiao CM, He MZ, Wang SM, Jiang XJ , Wang F , Li XJ, Tan S, Chao Z, Xin WS , Gao S , Yuan JL, Li Q, Xu ZC , Zheng XL, Zhao JG*, Liu GL *.Multi-omics analysis reveals substantial linkages between the oral-gut microbiomes and inflamm-aging molecules in elderly pigs. Frontiers in Microbiology. 2023: 15:14:1250891

6. Zhao Y, Chen CH ,Pan JL, Lam Sin Man , Shui GH, Yang SL, Wu TW , Yang N, Tao C, Zhao JG*; Wang, YF*. Adipocyte Rnf20 ablation increases the fast-twitch fibers of skeletal muscle via lysophosphatidylcholine 16:0. Cellular and Molecular Life Science.2023: 9;80(9):243.

7. Pan J, Chui L, Liu T, Zheng Q, Liu X, Liu L, Zhao Y, Zhang L, Song M, Han J, Huang J, Tang C, Tao C, Zhao JG*, Wang YF*. Fecal Microbiota Was Reshaped in UCP1 Knock-In Pigs via the Adipose-Liver-Gut Axis and Contributed to Less Fat Deposition. Microbiology Spectrum. 2023, 11 (1), e03540-22

8. Song R, Wang Y, Zheng Q, Yao J, Cao C, Wang Y*, Zhao JG*. One-step base editing in multiple genes by direct embryo injection for pig trait improvement. Sci China Life Sci. 2022 Ap;65(4):739-752.

9. Gu P, Hui X, Zheng Q, Gao Y, Jin L, Jiang W, Zhou C, Liu T, Huang Y, Liu Q, Nie T, Wang Y, Wang Y, Zhao JG*, Xu A*. Mitochondrial uncoupling protein 1 antagonizes atherosclerosis by blocking NLRP3 inflammasome-dependent interleukin-1β production. Sci Adv. 2021; 7(50): eabl4024.

10. Song R, Liu K, Wang Y, Qin G, Xiao M, Liu Y, Yang J, Zong R, Yan X, Zheng Q, Zhang H, Hou N, Yao J, Wang X, Wang Y, Zhao JG*, Zhang X*. A NCSTN heterozygous point mutation pig model simulates familial acne inversa and reveals dysregulated cholesterol biosynthesis via the notch-pAMPK-HMGCR pathway. Science Bulletin.2021, 66(23):2343-2346.

11. Yao J, Yu Wang Y, Cao C, Song R, Bi D, Zhang H, Li Y, Qin G, Hou N, Zhang N, Zhang J, Guo W, Yang S, Wang Y*, Zhao JG*. CRISPR/Cas9-Mediated Correction of MITF Homozygous Point Mutation in a Waardenburg syndrome 2A pig model. Molecular Therapy - Nucleic Acids. 2021, 24:986-999.

12. Liang X#, Tao C#, Pan J, Zhang L, Liu L, Zhao Y, Fan Y, Cao C, Liu J, Zhang J, Lam SM, Shui G, Jin W, Li W, Zhao JG*, Li K*, Wang Y*. Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis. Protein Cell. 2021, 12(6):475-492.

13. Song R, Wang Y, Wang Y* and Zhao JG*. Base editing in pigs for precision breeding. Front Agr Sci Eng. 2020, 7(2): 161–170, 2020.

14. Liu L, Wang Y, Liang X, Wu X, Liu J, Yang S, Tao C, Zhang J, Tian J, Zhao JG*, Wang Y*. Stearoyl-CoA desaturase is essential for porcine adipocyte differentiation. Int J Mol Sci. 2020 Apr 1;21(7): E2446.

15. Zhang Y, Hong Q, Cao C, Yang L, Li Y, Hai T, Zhang H, Zhou Q, Sui R, Zhao JG^*. A novel porcine model reproduces human oculocutaneous albinism type II. Cell Discov.2019 Oct 8;5:48.

16. Wang X, Cao C, Li Y, Hai T, Jia Q, Zhang Y, Zheng Q, Yao J, Qin G, Zhang H, Song R, Wang Y, Shui G, Lam SM, Liu Z, Wei H, Meng A, Zhou Q, Zhao JG^*.A harlequin ichthyosis pig model with a novel ABCA12 mutation can be rescued by acitretin treatment. J Mol Cell Biol. 2019 Mar 29. pii: mjz021. (Cover story)

17. Zhao JG, Lai L, Ji W, Zhou Q^*. Genome editing in large animals: Current status and future prospects. Natl Sci Rev.2019, 6(3) : 402–420

18. Cao C, Zhang Y, Jia Q, Wang X, Zheng Q, Zhang H, Song R, Li Y, Luo A, Hong Q, Qin G, Yao J, Zhang N, Wang Y, Wang H, Zhou Q, Zhao JG^*. An exonic splicing enhancer mutation in DUOX2 causes aberrant alternative splicing and severe congenital hypothyroidism in Bama pigs. Dis Model Mech.2019, 12: dmm036616

19. Wang Y, Huang JJ, Zhao JG^*. Gene engineering in swine for agriculture. J Integr Agr. 2017; 16(12): 2792-2804 (Invited Review)

20. Hai T, Guo W, Yao J, Cao C, Luo A, Qi M, Wang X, Wang X. Huang J, Zhang Y, Wang D, Shang H, Hong Q, Zhang R, Jia Q, Zheng Q, Qin G, Li Y, Zhang T, Jin W, Chen ZY, Wang H, Zhou Q, Meng A, Wei H*, Yang S*, Zhao JG*. Creation of miniature pig model of human Waardenburg syndrome type 2A by ENU mutagenesis. Human genetics. 2017; 136:1463-1475.

21. Zheng Q, Lin J, Huang J, Zhang H, Zhang R, Zhang X, Cao C, Hambly C, Qin G, Yao J, Song R, Jia Q, Wang X, Li Y, Zhang N, Piao Z, Ye R, Speakman JR, Wang H, Zhou Q, Wang Y, Jin W, Zhao JG*. Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decrease fat deposition and improve thermogenic capacity. Proc Natl Acad Sci U S A. 2017;

22. Zhang Y, Xue Y, Cao C, Huang J, Hong Q, Hai T, Jia Q, Wang X, Qin G, Yao J, Wang X, Zheng Q, Zhang R, Li Y, Luo A, Zhang N, Shi G, Wang Y, Ying H, Liu Z, Wang H, Meng A, Zhou Q, Wei H*, Liu F*, Zhao JG*. Thyroid hormone regulates hematopoiesis via the TR-KLF9 axis. Blood. 2017; 130(20):2161-2170.

23. Huang J, Wang Y, Zhao JG*. CRISPR editing in biological and biomedical investigation. J Cell Physiol. 2017; 9(5), 364-375.

24. Hai T, Cao C, Shang H, Guo W, Mu Y, Yang S, Zhang Y, Zheng Q, Zhang T, Wang X, Liu Y, Kong Q, Li K, Wang D, Qi M, Hong Q, Zhang R, Wang X, Jia Q, Wang X, Qin G, Li Y, Luo A, Jin W, Yao J, Huang J, Zhang H, Li M, Xie X, Zheng X, Guo K, Wang Q, Zhang S, Li L, Xie F, Zhang Y, Weng X, Yin Z, Hu K, Cong Y, Zheng P, Zou H, Xin L, Xia J, Ruan J, Li H, Zhao W, Yuan J, Liu Z, Gu W, Li M, Wang Y, Wang H*, Yang S*, Liu Z*, Wei H*, Zhao JG*, Zhou Q*, Meng A*. Pilot study of large-scale production of mutant pigs by ENU mutagenesis. Elife. 2017, 6. pii: e26248.

25. Lin J, Cao C, Tao C, Ye R, Dong M, Zheng Q, Wang C, Jiang X, Qin G, Yan C, Li K, Speakman JR, Wang Y*, Jin W*, Zhao JG*. Cold adaptation in pigs depends on UCP3 in beige adipocytes. J Mol Cell Biol. 2017 May 9.

26. Jia Q, Cao C, Tang H, Zhang Y, Zheng Q, Wang X, Zhang R, Wang X, Luo A, Wei H, Meng A, Zhou Q, Wang H*, Zhao JG*. A 2-bp insertion (c.67_68insCC) in MC1R causes recessive white coat color in Bama miniature pigs. J Genet Genomics. 2017,44(4):215-217.

27. Wang X, Cao C, Huang J, Yao J, Hai T, Zheng Q, Wang X, Zhang H, Qin G, Cheng J, Wang Y, Yuan Z, Zhou Q, Wang H*, Zhao JG*. One-step generation of triple gene targeted pigs using CRISPR/Cas9 system. Sci Rep. 2016 Accepted

28. Huang J, Zhang H, Yao J, Qin G, Wang F, Wang X, Luo A, Zheng Q, Cao C, Zhao JG*. BIX-01294 increases pig cloning efficiency by improving epigenetic reprogramming of somatic cell nuclei. Reproduction. 2016 Jan; 151(1):39-49.

29. Zhao W, Wang Y, Liu S, Huang J, Zhai Z, He C, Ding J, Wang J, Wang H, Fan W, Zhao JG*, Meng H*. The dynamic distribution of porcine microbiota across different ages and gastrointestinal tract segments. PLoS One. 2015 ; 10(2):e0117441. 2015. (*Co-corresponding Author)

30. Zhao JG , Xu WJ, Ross JW, Walters EM, Butler SP, Whyte JJ, Kelso L, Fatemi M, Vanderslice NC, Giroux K, Spate LD, Samuel MS, Murphy CN, Wells KD, Masiello NC, Prather RS& Velander WH. Engineering protein processing of the mammary gland to produce abundant hemophilia B therapy in milk. Sci Rep. 2015 Accepted.

31. Wang X, Zhou J, Cao C, Huang J, Hai T, Wang Y, Zheng Q, Zhang H, Qin G, Miao X, Wang H, Cao S*, Zhou Q*, Zhao JG*. Efficient CRISPR/Cas9-mediated biallelic gene disruption and site-specific knockin after rapid selection of highly active sgRNAs in pigs. Sci Rep. 2015 Aug 21;5:13348. (*Co-corresponding Author)

32. Huang J, Zhang H, Wang X, Dobbs KB, Yao J, Qin G, Whitworth KW, Walters EM, Prather RS*, Zhao JG*. Impairment of Preimplantation Porcine Embryo Development by Histone Demethylase KDM5B Knockdown Through Disturbance of Bivalent H3K4me3-H3K27me3 Modifications. Biol Reprod. 2015 (*Co-corresponding Author)

33. Yao J, Huang JJ, HaiTang , Wang XL, Qin GS, Zhang HY, Wu R, Xi J. J, Yuan Z, Zhao JG*. Efficient bi-allelic gene knockout and site-specific knock-in mediated by TALENs in pigs. Sci Rep. 2014 Nov 5, (*Corresponding Author)

34. Li G, Jia Q, Zhao J, Li X, Yu M, Samuel MS, Zhao S, Prather RS, Li C. Dysregulation of genome-wide gene expression and DNA methylation in abnormal cloned piglets. BMC Genomics.2014;15: 811.

35. Wang YP, Qi ST, Wei Y, Ge ZJ, Chen L, Hou Y, Ouyang YC, Schatten H, Zhao JG*, Sun QY*.Knockdown of UCHL5IP causes abnormalities in γ-tubulin localisation, spindle organisation and chromosome alignment in mouse oocyte meiotic maturation. Reprod Fertil Dev. 2013;25(3):495-502. (*Co-corresponding Author)

36. Whitworth KM, Zhao J, Spate LD, Li R, Prather RS.Scriptaid corrects gene expression of a few aberrantly reprogrammed transcripts in nuclear transfer pig blastocyst stage embryos. Cell Reprogram. 2011 13(3):191-204.

37. Whyte JJ*, Zhao JG*, Samuel M, Laughlin HM, Prather RS, Gene Targeting with Zinc Finger Nucleases to Produce Cloned eGFP Knockout Pigs. Mol Reprod Dev. 2011, 78(1):2 (*equal contribution)

38. Zhao JG, Whyte JJ, Prather RS. The Effect of Epigenetic Regulation on Somatic Cell Nuclear Transfer. Cell Tissue Res. 2010, 341(1):13-21. (invited review)

39. Zhao JG, Hao Y, Ross JW, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS. Histone Deacetylase Inhibitors Improve In Vitro and In Vivo Developmental Competence of Somatic Nuclei Nuclear Transfer Porcine Embryos. Cell Reprogram.2010;12(1):75-83

40. Zhao J, Ross JW, Hao Y, Spate LD, Walters EM, Samuel MS, Rieke A, Murphy CN, Prather RS. Significant Improvement in Cloning Efficiency of an Inbred Miniature Pig by Histone Deacetylase Inhibitor Treatment after Somatic Cell Nuclear Transfer. Biol Reprod. 2009, 81: 525-530

41. Zhao JG, Li H, Kong X, Tang ZQ. Association analysis of SNPs in the chicken UCP gene with growth and body composition traits. Canadian Journal of Animal Science. 2006, 86:345-350

42. Zhao JG, Li H, Wang YX, Meng H. The expression characterization of chicken uncoupling protein gene. Asian-Australasian Journal of Animal Science.005, 18 (11): 1552-1556

43. Yang XY, Zhao JG, Li HW, Li H, Liu HF, Huang SZ, Zeng YT. Improve the in vitro development of cloned bovine embryos by use of holstein-chinese yellow hybrid recipient oocytes recovered by ovum pick up. Theriogenology. 2005, 64(6):1263-72

44. Zhao JG, Li H, Wang QG, Wang YX,Tang ZQ. Cloning and sequencing of the introns of UCP gene and construction of molecular phylogenetic tree in chicken. Hereditas (Beijing).04,26(1):50-54 (Abstract in English)

45. Zhao JG, Li H, Meng H, Gu ZL, Wang QG, Wang YX. Study on the uncoupling protein gene as the candidate gene for fatness traits in chicken. Acta Genetica Sinica.2002, 29(6): 481-486 (Abstract in English)

【专利成果】

[1] 赵建国, 张颖, 戚亚男, 田双杰, 宗若君, 闫星慧, 王煜. 一种引导编辑系统和目的基因序列的定点修饰方法. CN: CN116716298A, 2023-09-08.

[2] 赵建国, 王霄, 宋瑞高, 刘强, 秦国嵩, 姚婧, 张颖. 一种猪TMC1突变基因及其用途. CN: CN115181170A, 2022-10-14.

[3] Jianguo Zhao, Qiantao Zheng, Guosong Qin, Jing Yao, Chunwei Cao. Cold-resistant and lean-type transgenic pig and preparation method therefor. CN: US11419320B2, 2022-08-23.

[4] 赵建国, 孙泽文, 张颖, 王霄, 秦国嵩, 姚婧. 突变基因及其用途. CN: CN114181944A, 2022-03-15.

[5] 赵建国, 王霄, 曹春伟, 海棠, 周琪, 王红梅, 张颖, 贾启涛, 郑千涛. 一种突变基因及其用于构建斑色鱼鳞癣小型猪模型的用途. CN: CN110846321B, 2021-11-19.

[6] 周琪, 赵建国, 海棠, 贾启涛, 曹春伟, 王红梅, 张颖, 郑千涛, 王霄. 一种突变基因及其用于构建蹼足病小型猪模型的用途. CN: CN110846330B, 2021-08-27.

[7] 郑爱华, 赵超越, 李虹悦, 张毓航, 金万洙, 赵建国. 基于水疱性口炎病毒载体的重组新型冠状病毒及其制备方法与应用. CN: CN112941038A, 2021-06-11.

[8] 赵建国. 突变基因及构建短肢性侏儒症小型猪模型的方法和用途. ZL202010959862.2, 2020-09-14.

[9] 赵建国, 郑千涛, 黄娇娇, 秦国嵩, 金万洙. 一种抗寒及瘦肉型转基因猪及其制备方法. CN: CN107182940A, 2017-09-22.

[10] 王红梅, 周琪, 赵建国, 孟安明, 曹春伟, 海棠, 祁蒙. 一种猪MITF基因突变的检测方法. CN: CN107034268B, 2017-08-11.

[11] 赵建国, 姚婧, 黄娇娇, 秦国嵩. 一种敲除猪DJ-1基因的细胞的构建方法及其应用. CN: CN107034192A, 2017-08-11.

[12] 何宏轩, 王玉田, 王承民, 贾亚雄, 马志军, 赵建国. 一种消毒剂及其制备方法和用途. CN: CN105519588A, 2016-04-27.